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Among Antibody-Like Molecules, Monobodies, Able to Interact with Nucleocapsid Protein of SARS-CoV Virus, There Are Monobodies with High Affinity to Nucleocapsid Protein of SARS-CoV-2 Virusстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 25 мая 2022 г.
- Авторы: Khramtsov Y.V., Ulasov A.V., Lupanova T.N., Georgiev G.P., Sobolev A.S.
- Журнал: Doklady Biochemistry and Biophysics
- Том: 503
- Год издания: 2022
- Издательство: M A I K Nauka - Interperiodica
- Местоположение издательства: Russian Federation
- Первая страница: 90
- Последняя страница: 92
- DOI: 10.1134/S1607672922020077
- Аннотация: Seven amino acid sequences of antibody mimetics molecules, monobodies, capable of interacting with the nucleocapsid protein of the SARS-CoV virus, were taken from the literature. Nucleotide sequences of monobody genes were obtained by gene synthesis, which were expressed in E. coli and isolated using Ni- NTA chromatography. It was shown by thermophoresis that three of the seven selected antibody-like mole- cules can interact with high affinity (dissociation constant of tens of nM) with the nucleocapsid protein of the SARS-CoV-2 virus. For the remaining four monobodies, only low affinity binding with a dissociation con- stant of several μM was found.
- Добавил в систему: Соболев Александр Сергеевич