RHAMM-Target Peptides as Molecular Imaging Probes for the Imaging of RHAMM-Expressing Cancer Cells

Akentieva, N.P.; Topunov, A.F.

Авторы: Akentieva Natalia P., Topunov Alexey F.
Журнал: Biointerface Research in Applied Chemistry
Том: 12
Номер: 1
Год издания: 2021
Первая страница: 2
Последняя страница: 24
DOI: 10.33263/briac121.002024
Аннотация: The incidence of cancer in the world is growing steadily. Therefore, it is necessary to developnew approaches for the early diagnosis of cancer. This work is devoted to the study of the potential ofRHAMM-target peptides for molecular diagnosis of cancer. The key amino acids of the RHAMM targetpeptides were identified by the alanine scan method. The specificity of binding of peptides to RHAMMCTwas assessed using competitive HA substitution by the ELISA method. RHAMM-CT was obtainedby genetic engineering and isolated by affinity chromatography. The interaction of RHAMM targetpeptides with the surface receptor of tumor cells was evaluated by confocal microscopy. It has beenshown that fragment EEGEEZ in the peptides' composition is necessary for binding to the RHAMMCT.The results showed that the RHAMM-target peptides bind specifically to the RHAMM-CT andcompetitively substituted HA at the RHAMM. It has been found that aggrecan is unable to displacepeptides from the HA binding site of RHAMM-CT. The results showed that the FITC peptide bindsspecifically to RHAMM on the surface of prostate cancer cells. Therefore, RHAMM-target peptideshave the potential for early molecular diagnosis of cancer.
Добавил в систему: Алдошин Сергей Михайлович

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RHAMM-Target Peptides as Molecular Imaging Probes for the Imaging of RHAMM-Expressing Cancer Cellsстатья

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