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Synthesis of α-D-Ribose 1-Phosphate and 2-Deoxy-α-D-Ribose 1-Phosphate Via Enzymatic Phosphorolysis of 7-Methylguanosine and 7-Methyldeoxyguanosineстатья
- Авторы: Varizhuk Irina V., Oslovsky Vladimir E., Solyev Pavel N., Drenichev Mikhail S., Mikhailov Sergey N.
- Журнал: Current protocols in nucleic acid chemistry
- Том: 2
- Номер: 1
- Год издания: 2022
- Издательство: Wiley-Liss Inc
- Местоположение издательства: United States
- Номер статьи: e347
- DOI: 10.1002/cpz1.347
- Аннотация: A simple and efficient method for the preparation of α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near irreversible enzymatic phosphorolysis of readily prepared hydroiodide salts of 7-methylguanosine and 7-methyl-2'-deoxyguanosine respectively in the presence of purine nucleoside phosphorylase. α-D-Ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate are obtained in near quantitative yields (by HPLC analysis) and 74-94% yields after their isolation and purification.
- Добавил в систему: Сольев Павел Николаевич