Efficient Assay and Marker Significance of NAD+ in Human Blood

Balashova, N.V.; Zavileyskiy, L.G.; Artiukhov, A.V.; Shaposhnikov, L.A.; Sidorova, O.P.; Tishkov, V.I.; Tramonti, A.; Pometun, A.A.; Bunik, V.I.

Авторы: Balashova Natalia V., Zavileyskiy Lev G., Artiukhov Artem V., Shaposhnikov Leonid A., Sidorova Olga P., Tishkov Vladimir I., Tramonti Angela, Pometun Anastasia A., Bunik Victoria I.
Журнал: Frontiers in Medicine
Том: 9
Год издания: 2022
Первая страница: 1
Последняя страница: 8
Номер статьи: 886485
DOI: 10.3389/fmed.2022.886485
Аннотация: Nicotinamide adenine dinucleotide (NAD+) is a biological molecule of systemic importance. Essential role of NAD+ in cellular metabolism relies on the substrate action in various redox reactions and cellular signaling. This work introduces an efficient enzymatic assay of NAD+ content in human blood using recombinant formate dehydrogenase (FDH, EC 1.2.1.2) and demonstrates its diagnostic potential, comparing NAD+ content in the whole blood of control subjects and patients with cardiac and neurologic pathologies. In the control group (n=22, 25-70 years old), our quantification of the blood concentration of NAD+ (18 M, minimum 15, max 23) corresponds well the NAD+ quantifications reported in literature. In patients with neurological diseases (n=10, 18-55 years old), the NAD+ level significantly (p<0.0001) decreases (to 14 M, min 13, max 16), compared to the control group. In cardiac patients (n=24, 42-83 years old), the blood levels of NAD+ (13 M, min 9, max 18) are lower than those in the control subjects (p<0.0001) or neurological patients (p=0.1). A better discrimination of the cardiac and neurological patients is achieved when the ratios of NAD+ to age, blood creatinine or mean corpuscular volume are compared. The proposed NAD+ assay provides an easy and robust tool for clinical analyses of this important metabolic indicator, whose blood levels may discriminate the control subjects, neurological patients and cardiac patients and which is applicable for better diagnostics of other systemic pathologies. Following the NAD+ dynamics in critically ill patients may help assessing the state of a patient and efficiency of therapeutic approaches
Добавил в систему: Тишков Владимир Иванович

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