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Compaction and transmembrane delivery of pDNA: differences between l-PEI and two types of amphiphilic block copolymersстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 апреля 2017 г.
- Авторы: Raup A., Wang H., Synatschke C.V., Jérôme V., Agarwal S., Pergushov D.V., Müller A.H.E, Freitag R.
- Журнал: Biomacromolecules
- Том: 18
- Номер: 3
- Год издания: 2017
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 808
- Последняя страница: 818
- DOI: 10.1021/acs.biomac.6b01678
- Аннотация: Polycations are popular agents for non-viral delivery of DNA to mammalian cells. Adding hydrophobic, biodegradable, or cell penetrating functions could help to improve their performance, which at present is below that of viral agents. A crucial first step in gene delivery is the complexation of the DNA. The characteristics of these ‘polyplexes’ presumably influence or even determine the subsequent steps of membrane passage, intracellular travelling / DNA release, and nuclear up-take. Herein, polyplexes formed with linear poly(ethylenimine) (l-PEI) are compared to complexes generated with functionalized diblock copolymers. While l-PEI interacts only electrostatically with the DNA, interaction in case of the diblock polymers may be mixed-mode. In certain cases transfection efficiency improved when the polyplexes were formed in hypertonic solution. Moreover, whereas conventional PEI-based polyplexes enter the cells via endocytosis, at least one of the diblock agents seemed to promote entry via transient destabilization of the plasma membrane.
- Добавил в систему: Пергушов Дмитрий Владимирович