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Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tuстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 13 июля 2022 г.
- Авторы: Pletnev Philipp I., Shulenina Olga, Evfratov Sergey, Treshin Vsevolod, Subach Maksim F., Serebryakova Marina V., Osterman Ilya A., Paleskava Alena, Bogdanov Alexey A., Dontsova Olga A., Konevega Andrey L., Sergiev Petr V.
- Журнал: Journal of Biological Chemistry
- Том: 298
- Номер: 5
- Год издания: 2022
- Издательство: American Society for Biochemistry and Molecular Biology Inc.
- Местоположение издательства: United States
- Первая страница: 101914
- DOI: 10.1016/j.jbc.2022.101914
- Аннотация: -terminal acetylation is widespread in the eukaryotic proteome but in bacteria is restricted to a small number of proteins mainly involved in translation. It was long known that elongation factor Tu (EF-Tu) is N-terminally acetylated, whereas the enzyme responsible for this process was unclear. Here, we report that RimI acetyltransferase, known to modify ribosomal protein S18, is likewise responsible for N-acetylation of the EF-Tu. With the help of inducible tufA expression plasmid, we demonstrated that the acetylation does not alter the stability of EF-Tu. Binding of aminoacyl tRNA to the recombinant EF-Tu in vitro was found to be unaffected by the acetylation. At the same time, with the help of fast kinetics methods, we demonstrate that an acetylated variant of EF-Tu more efficiently accelerates A-site occupation by aminoacyl-tRNA, thus increasing the efficiency of in vitro translation. Finally, we show that a strain devoid of RimI has a reduced growth rate, expanded to an evolutionary timescale, and might potentially promote conservation of the acetylation mechanism of S18 and EF-Tu. This study increased our understanding of the modification of bacterial translation apparatus.
- Добавил в систему: Серебрякова Марина Васильевна