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The effect of cellobiohydrolase 1 gene knockout for composition and hydrolytic activity of the enzyme complex secreted by filamentous fungus Penicillium verruculosumстатья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 25 мая 2022 г.
- Авторы: Kislitsin Valeriy Yu, Chulkin Andrey M., Zorov Ivan N., Denisenko Yuri, Sinitsyn Arkadiy P., Rozhkova Alexandra M.
- Журнал: Bioresource Technology Reports
- Том: 18
- Год издания: 2022
- Издательство: Elsevier
- Местоположение издательства: United States
- Первая страница: 1
- Последняя страница: 8
- Номер статьи: 101023
- DOI: 10.1016/j.biteb.2022.101023
- Аннотация: The filamentous fungus Penicillium verruculosum is used as an industrial producer of cellulases and other enzymes and enzymatic complexes. Secreted cellobiohydrolases (CBHs) are the key components of the P. verruculosum enzyme complex because they can hydrolyse both amorphous and microcrystalline cellulose (MCC). CBHs cleave cellobiose residues from the ends of polymer molecules of native or partially hydrolysed cellulose. There are two CBH isoforms: CBH 1 (EC 3.2.1.176) attacks the cellulose molecule from the reducing end, while CBH 2 (EC 3.2.1.91) acts from the non-reducing end. In this study, the cbh1 gene encoding CBH 1 was knocked out by adapted CRISPR/Cas9 genome editing technology. As a result of the СBH 1 removal, the content of CBH 2, endoglucanase (EG), xylanase and β-glucosidase (BG) increased. However, this compensatory effect did not lead to an increase in hydrolytic efficiency in the saccharification of microcrystalline cellulose and pretreated aspen wood.
- Добавил в систему: Зоров Иван Никитич