Methodology of Purification of Inactivated Cell-Culture-Grown SARS-CoV-2 Using Size-Exclusion Chromatography

Авторы: Kovpak Anastasia A., Piniaeva Anastasia N., Gerasimov Oleg A., Tcelykh Irina O., Ermakova Mayya Y., Zyrina Anna N., Danilov Dmitry V., Ivin Yury Y., Kozlovskaya Liubov I., Ishmukhametov Aydar A.
Журнал: VACCINES
Том: 10
Номер: 949
Год издания: 2022
DOI: 10.3390/vaccines10060949
Аннотация: Various types of COVID-19 vaccines, including adenovirus, mRNA, and inactivated ones,have been developed and approved for clinical use worldwide. Inactivated vaccines are producedusing a proven technology that is widely used for the production of vaccines for the prevention andcontrol of infectious diseases, including influenza and poliomyelitis. The development of inactivatedwhole-virion vaccines commonly includes several stages: the production of cellular and viral biomassin cell culture; inactivation of the virus; filtration and ultrafiltration; chromatographic purification ofthe viral antigen; and formulation with stabilizers and adjuvants. In this study, the suitability of fourresins for Size-Exclusion Chromatography was investigated for the purification of a viral antigen forthe human COVID-19 vaccine.
Добавил в систему: Зырина Анна Николаевна

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