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Analysis of binding specificity of disulfide bonded dimeric lambda-Cro V55C protein with generic hexamer oligonucleotide microchipстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 октября 2013 г.
- Авторы: Chechetkin VR, Prokopenko DV, Zasedateleva OA, Gitelson GI, Lomakin ES, Livshits MA, Malinina L., Turygin AY, Krylov AS, Mirzabekov AD
- Журнал: Journal of Biomolecular Structure and Dynamics
- Том: 21
- Номер: 3
- Год издания: 2003
- Издательство: Adenine Press
- Местоположение издательства: United States
- Первая страница: 425
- Последняя страница: 433
- DOI: 10.1080/07391102.2003.10506937
- Аннотация: Binding specificity of mutant V55C disulfide bonded dimeric lambda-Cro protein (CroVC) to double-stranded DNA (dsDNA) was studied using generic hexamer oligonucleotide microchip. The curves of dissociation of hybridized DNA in the presence and absence of CroVC were converted into the effective discriminant constants to assess the relevant thermodynamic equilibrium binding constants for dsDNA-protein complexes. Then, tiling of longer oligonucleotides with shorter oligomers was used to search for sequence motifs with the highest binding specificity similarly to sequencing by hybridization. The comparison of the deduced sequences with the known natural operator half-sites demonstrated the principal ability to discern and reconstruct the major parts of 7-mer motifs corresponding to the strongest binding of CroVC subunits. Our results show the applicability of generic microchips to the analysis of binding specificity in the case of multi-subunit DNA-binding proteins.
- Добавил в систему: G.I.Gitelson