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Oxidation mimicking substitution of conservative cysteine in recoverin suppresses its membrane associationстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Permyakov Sergei E., Zernii Evgeni Yu, Knyazeva Ekaterina L., Denesyuk Alexander I., Nazipova Aliya A., Kolpakova Tatiana V., Zinchenko Dmitry V., Philippov Pavel P., Permyakov Eugene A., Senin Ivan I.
- Журнал: Amino Acids
- Том: 42
- Номер: 4
- Год издания: 2012
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 1435
- Последняя страница: 1442
- DOI: 10.1007/s00726-011-0843-0
- Аннотация: Recoverin belongs to the family of intracellular Ca2+-binding proteins containing EF-hand domains, neuronal calcium sensors (NCS). In photoreceptor outer segments, recoverin is involved into the recovery of visual cycle via Ca2+-dependent interaction with disk membranes and inhibition of rhodopsin kinase. The function of a conservative within NCS family Cys residue in the inactive EF-loop 1 remains unclear, but previous study has shown its vulnerability to oxidation under mild oxidizing conditions. To elucidate the influence of oxidation of the conservative Cys39 in recoverin the properties of its C39D mutant, mimicking oxidative conversion of Cys39 into sulfenic, sulfinic or sulfonic acids have been studied using intrinsic fluorescence, circular dichroism, and equilibrium centrifugation methods. The C39D substitution results in essential changes in structural, physico-chemical and physiological properties of the protein: it reduces alpha-helical content, decreases thermal stability and suppresses protein affinity for photoreceptor membranes. The latter effect precludes proper functioning of the Ca2+-myristoyl switch in recoverin. The revealed significance of oxidation state of Cys39 for maintaining the protein functional status shows that it may serve as redox sensor in vision and suggests an explanation of the available data on localization and light-dependent translocation of recoverin in rod photoreceptors.
- Добавил в систему: Зерний Евгений Юрьевич