Isolation of the constitutive heterochromatin from mouse liver nucleiстатья
Глава в книге
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
Авторы:
Zatsepina O.V. ,
Zharskaya O.O.,
Prusov A.N.
Журнал:
Methods in molecular biology (Clifton, N.J.)
Том:
463
Год издания:
2008
Издательство:
Humana Press, Inc.
Местоположение издательства:
United States
Первая страница:
169
Последняя страница:
180
DOI:
10.1007/978-1-59745-406-3-12
Аннотация:
A method for isolation of constitutive heterochromatin (chromocenters) from nuclei of mouse liver cells is described. This method is based on the higher resistance of chromocenters to low ionic strength treatment as compared with that of nucleoli and euchromatin. The method allows separation of chromocenters that are essentially free of nucleoli and other nuclear contaminants. In contrast to nuclei and nucleoli, isolated chromocenters are characterized by a simpler protein composition and contain a smaller number of proteins (especially of high molecular weight proteins). They possess telomeric DNA and telomerase activity that suggests a tight association of chromocenters with the telomerase complex in mouse hepatocyte nuclei. В© 2008 Humana Press.
Добавил в систему:
Прусов Андрей Николаевич