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Measurements of the light-induced steady state electric potential generation by photosynthetic pigment-protein complexesстатья Исследовательская статья
- Авторы: Mamedov M.D., Milanovsky G.E., Vitukhnovskaya L., Semenov A.Yu
- Журнал: Biophysical Reviews
- Том: 14
- Год издания: 2022
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 933
- Последняя страница: 939
- DOI: 10.1007/s12551-022-00966-2
- Аннотация: In this minireview, we consider the methods of measurements of the light-induced steady state transmembrane electric potential (Δψ) generation by photosynthetic systems, e.g. photosystem I (PS I). The microelectrode technique and the detection of electrochromic bandshifts of carotenoid pigments are most appropriate for Δψ measurements in situ and in vivo. Direct electrometrical method and Δψ measurements in the photovoltaic system based on membrane filter (MF) sandwiched between semiconductor indium tin oxide electrodes (ITO) are suitable for studies of isolated pigment-protein complexes and small natural vesicles—chromatophores. In the presence of trehalose, ITO|PS I-MF|ITO system allows to keep a steady state level of ∆ψ after 1 h of illumination. According to preliminary experiments, this system is capable of providing steady state light-induced ∆ψ after several months of storage in the dark at room temperature under controlled humidity in the presence of trehalose. The long-term generation of light-induced ∆ψ in relatively simple system may serve as a source of the solar-to-electric energy conversion.
- Добавил в систему: Милановский Георгий Евгеньевич