Divergence of repetitive sequences of DNA in echinoderms .1. comparison of sequences with a high degree of intragenome divergenceстатья
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Аннотация:The molecular DNA-DNA hybrization method was used for comparison of repetitive sequences with a high degree of intragenomic divergence in sevaen species of Echinodermata, representing of three classes of this phyla, and also for repesentative of the phyla Annelida. The value of of intragenomic divergence was estimated with respect to a thermolabile fraction of repetitive [3H]DNA of Strongylocentrotus intermedius isolated by thermoelution at 70o after reassotian up to Cot = 140. The data obtained indicate a significant conservatism of the given fraction: its homologues are presen in DNA of all the species studied. Hybridization of the thermolabile fraction of [3H]DNA S. intermedius at Cot = 10 and 1000 allowed one to estimate the divergence of sequeces in DNA of all the species studied. Hybridization of the thermolabile fraction of [3H]DNA highly repetitive sequences of the given DNA fraction is more appreciable than that of moderately repetitive ones. Intagenomic divergence of moderately repetitve thermolabile sequences is higher than that of highly repetitive sequences both in phylogenetically close and distant species.