The genome of the pogonophoran Siboglinum fiordicum - characteristics of its organization and divergence from the genomes of several representative invertebrate animalsстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 октября 2013 г.
Аннотация:DNA of Pogonophora Siboglinum fiordicum isolated from ethanol fixed animals was studied. It contains 40.5% GC according to the melting temperature and 39.3% GC as estimated by cation-exchange chromatography. From the reassociation kinetic data with a [125I] tracer 400 nucleotide long it is clear that 70% of the genome consists of repetitive sequences. The fast component (Cot < 0.04) consitites 11% of the genome, the intermediate (Cot1/2, repetition frequency 1600) - 59%. The unique sequences comprise about 30% of the genome. Experiments where [125I] tracers of increasing length were reassociated with driver DNA fragments 300 nucleotides long point out that at least 80% genome contains repetitive sequences part of them being organized in short-period interspersion patterns. The hybridization of Pogonophora [125I]DNA fragments with DNA of invertebrates of other phyla - Echinodermata, Ascidia, Mollusca, Annelida - did not show any measurable homology between their DNA neither in repetitive nor in the unique fractions.