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Photobiology of lipofuscin granules in the retinal pigment epithelium cells of the eye: norm, pathology, ageстатья
- Авторы: Feldman T.B., Dontsov A.E., Yakovleva M.A., Ostrovsky M.A.
- Журнал: Biophysical Reviews
- Том: 14
- Год издания: 2022
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 1051
- Последняя страница: 1065
- DOI: 10.1007/s12551-022-00989-9
- Аннотация: Lipofuscin granules (LGs) are accumulated in the retinal pigment epithelium (RPE) cells. The progressive LG accumulation can somehow lead to pathology and accelerate the aging process. The review examines composition, spectral properties and photoactivity of LGs isolated from the human cadaver eyes. By use of atomic force microscopy and near-feld microscopy, we have revealed the fuorescent heterogeneity of LGs. We have discovered the generation of reactive oxygen species by LGs, and found that LGs and melanolipofuscin granules are capable of photoinduced oxidation of lipids. It was shown that A2E, as the main fuorophore (bisretinoid) of LGs, is much less active as an oxidation photosensitizer than other fuorophores (bisretinoids) of LGs. Photooxidized products of bisretinoids pose a much greater danger to the cell than non-oxidized one. Our studies of the fuorescent properties of LGs and their fuorophores (bisretinoids) showed for the frst time that their spectral characteristics change (shift to the short-wavelength region) in pathology and after exposure to ionizing radiation. By recording the fuorescence spectra and fuorescence decay kinetics of oxidized products of LG fuorophores, it is possible to improve the methods of early diagnosis of degenerative diseases. Lipofuscin (“aging pigment”) is not an inert “slag”. The photoactivity of LGs can pose a signifcant danger to the RPE cells. Fluorescence characteristics of LGs are a tool to detect early stages of degeneration in the retina and RPE.
- Добавил в систему: Фельдман Татьяна Борисовна