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A new enzyme preparation containing polysaccharide monooxygenase and beta-glucosidase — synergistic additives to cellulasesстатья
- Авторы: Semenova M.V., Gusakov A.V., Telitsin V.D., Matys V.Y., Bubnova T.V., Nemashkalov V.A., Rozhkova A.M., Sinitsyn A.P.
- Журнал: Applied Biochemistry and Microbiology
- Том: 58
- Номер: 4
- Год издания: 2022
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 449
- Последняя страница: 455
- DOI: 10.1134/s0003683822040147
- Аннотация: Based on the recipient strain of Penicillium verruculosum B1-537 (ΔniaD) using the promoter of the cellobiohydrolase I gene, a producer of homologous lytic polysaccharide monooxygenase (PMO) and heterologous β-glucosidase Aspergillus niger (BG), auxiliary enzymes for the “basic” cellulase complex consisting of endoglucanases and cellobiohydrolase, was obtained. The enzyme preparation PMO–BG was obtained, containing 34% PMO and 43% BG, which was used as a source of auxiliary enzymes that increase the effectiveness of basic cellulases P. verruculosum by 20–100% in the bioconversion of various types of cellulose-containing raw materials: microcrystalline cellulose, pretreated with alkali common reed and semi-bleached sulfate hardwood pulp of Arkhangelsk Pulp and Paper Mill JSC.
- Добавил в систему: Гусаков Александр Васильевич