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The charge and phase state of liposomes dramatically affects the binding of mannosylated chitosanстатья Исследовательская статья
- Авторы: Le-Deygen Irina, Rokosovina Viktoria, Skuredina Anna, Yakimov Ivan, Kudryashova Elena
- Журнал: Future Pharmacology
- Том: 2
- Номер: 3
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- DOI: 10.3390/futurepharmacol2030023
- Аннотация: Liposomal complex with mucoadhesive polymers e.g. mannosylated chitosan are considered as a perspective antituberculosis drugs delivery systems. The properties of such complexes can be critically affected by the charge and phase state of liposomes. The aim of our work is to study the interaction of mannosylated chitosan with liposomes of various compositions and to identify the key patterns of this process. We tracked interaction by titrating the liposomes with an increasing base-molar excess using the DLS method. Sorption isotherms were obtained using ATR-FTIR spectroscopy and linearized in the Sketchard coordinates to evaluate Kdis. The inclusion of cardiolipin in the lipid composition helps to reduce the dissociation constant of the complexes by an order of magnitude (3.8*10-4 M and 6.4*10-5 M for DPPC and DPPC:CL 80:20, respectively). Preheating at 37°C of gel-like anionic liposomes helps to reduce the Kdis to 3.5*10-5 M. Anionic liposomes both in liquid crystal and in the gel-like state form multipoint non-covalent complexes with chitosan-mannose conjugates due to partial neutralization of charges on the surface of vesicles, while neutral liposomes both states form unstable heterogeneous complexes, probably due to the predominant sorption of the polymer on vesicles.
- Добавил в систему: Ле-Дейген Ирина Михайловна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | futurepharmacol-1820531.pdf | 1,3 МБ | 20 августа 2022 [i.m.deygen] | |
| 2. | Сертификат о принятии к публикации | Acceptance-Certificate-futurepharmacol-1820531_1.pdf | 101,6 КБ | 20 августа 2022 [i.m.deygen] |