|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
New Amphiphilic Branched Copolymers of N-Vinylpyrrolidone with Methacrylic Acid for Biomedical Applicationsстатья
- Авторы: Kurmaz S.V., Ivanova I.I., Fadeeva N.V., Perepelitsina E.O., Lapshina M.A., Balakina A.A., Terent’ev A.A.
- Журнал: Polymer Science, Series A
- Год издания: 2022
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- DOI: 10.1134/S0965545X22700237
- Аннотация: Amphiphilic copolymers of N-vinylpyrrolidone and methacrylic acid, and triethylene glycoldimethacrylate (branching agent) are synthesized by radical copolymerization in toluene and ethanol; their main physicochemical characteristics (monomer composition, absolute weight-average molecular weight,polydispersity, and hydrodynamic radius in water and aqueous media with various рН) are determined. It is shown that in weakly acidic media copolymer solutions exhibit the lower critical temperature which shifts tohigh values in neutral and alkaline aqueous buffered solutions. Water-soluble nanoscale systems of red-fluorescent dye zinc tetraphenylporphyrinate having a hydrodynamic radius of about 50 nm (in aqueous neutralbuffered solution) and stable in the physiologically important temperature range are obtained. Using the МТТ assay on various cell lines, normal (FetMSC, Vero) and tumor (HepG2, HeLa), ternary copolymersare found to be low toxic. The dynamics of accumulation of dye-loaded polymer particles in HeLa and Vero cells is studied by f luorescence microscopy. It is concluded that the synthesized copolymers show promise ascarriers and means of delivery of biologically active compounds.
- Добавил в систему: Курмаз Светлана Викторовна