Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project

Volynkina, I.A.; Zakalyukina, Y.V.; Alferova, V.A.; Belik, A.R.; Yagoda, D.K.; Nikandrova, A.A.; Buyuklyan, Y.A.; Udalov, A.V.; Golovin, E.V.; Kryakvin, M.A.; Lukianov, D.A.; Biryukov, M.V.; Sergiev, P.V.; Dontsova, O.A.; Osterman, I.A.

Авторы: Volynkina Inna A., Zakalyukina Yuliya V., Alferova Vera A., Belik Albina R., Yagoda Daria K., Nikandrova Arina A., Buyuklyan Yuliya A., Udalov Andrei V., Golovin Evgenii V., Kryakvin Maxim A., Lukianov Dmitrii A., Biryukov Mikhail V., Sergiev Petr V., Dontsova Olga A., Osterman Ilya A.
Журнал: Antibiotics
Том: 11
Номер: 9
Год издания: 2022
Издательство: MDPI Publishing
Местоположение издательства: Basel, Switzerland, Switzerland
Первая страница: 1
Последняя страница: 17
Номер статьи: 1198
DOI: 10.3390/antibiotics11091198
Аннотация: Since the discovery of streptomycin, actinomycetes have been a useful source for new antibiotics, but there have been diminishing rates of new finds since the 1960s. The decreasing probability of identifying new active agents led to reduced interest in soil bacteria as a source for new antibiotics. At the same time, actinomycetes remain a promising reservoir for new active molecules. In this work, we present several reporter plasmids encoding visible fluorescent protein genes. These plasmids provide primary information about the action mechanism of antimicrobial agents at an early stage of screening. The reporters and the pipeline described have been optimized and designed to employ citizen scientists without specialized skills or equipment with the aim of essentially crowdsourcing the search for new antibiotic producers in the vast natural reservoir of soil bacteria. The combination of mechanism-based approaches and citizen science has proved its effectiveness in practice, revealing a significant increase in the screening rate. As a proof of concept, two new strains, Streptomyces sp. KB-1 and BV113, were found to produce the antibiotics pikromycin and chartreusin, respectively, demonstrating the efficiency of the pipeline.
Добавил в систему: Волынкина Инна Александровна

	ИСТИНА	Войти в систему Регистрация
	ИСТИНА ИНХС РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИСТИНА ИНХС РАН

Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Projectстатья Исследовательская статья

Прикрепленные файлы