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The Use of Capillaroscopy and Aggregometry Methods to Diagnose the Alterations of Microcirculation and Microrheology in Diabetes. In: Biomedical Photonics for Diabetes Research, 1st Edition. Edited by Andrey Dunaev and Valery Tuchinстатья Электронная публикация
- Авторы: Lugovtsov Andrei E., Gurfinkel Yury I., Ermolinskiy Petr B., Fabrichnova Anastasia A., Priezzhev Alexander V.
- Сборник: Biomedical Photonics for Diabetes Research
- Год издания: 2022
- Издательство: CRC Press
- Местоположение издательства: Boca Raton, United States
- DOI: 10.1201/9781003112099
- Аннотация: In 2021, over 537 million people worldwide were diagnosed with diabetes, according to the International Diabetes Federation and so the diagnosis, care and treatment of patients with diabetes mellitus have become one of the highest healthcare priorities. Biomedical photonics methods have been found to significantly improve and assist in the diagnosis of various disorders and complications arising from diabetes. These methods have also been widely used in various studies in the field of diabetes, including in the assessment of biochemical characteristics, metabolic processes, and microcirculation that are impaired in this disease.This book provides an introduction to methods of biomedical photonics. The chapters, written by world-leading experts, cover a wide range of issues, including the theoretical basis of different biophotonics methods and practical issues concerning the conduction of experimental studies to diagnose disorders associated with diabetes. It provides a comprehensive summary of the recent advances in biomedical optics and photonics in the study of diabetes and related complications.This book will be of interest to biomedical physicists and researchers, in addition to practicing doctors and endocrinologists looking to explore new instrumental methods for monitoring the effectiveness of patient treatment.In 3rd chapter, we review the principles and capabilities of optical techniques based on the interaction of light with whole-blood or red blood cell (RBC) suspensions to assess the parameters of blood microcirculation and microrheology. Several methods providing in vitro measurements of microrheologic parameters and in vivo monitoring of blood microcirculation are discussed. The former methods include aggregation and deformability properties of RBCs, forces of their pair interaction, and aggregation rate measured by diffuse light scattering, laser diffractometry, and laser trapping and manipulation techniques. Digital vital capillaroscopy for the estimation of the blood capillary velocity, the presence or absence of aggregates, and stasis in nail bed terminal capillaries is outlined. Special attention is paid to the alterations of these RBC properties in patients suffering from type 2 diabetes mellitus (DM) without and with concomitant diseases. Next, we show that in patients with DM, the ability of RBCs to deform is slightly reduced while the aggregation rate and forces of cell interaction are significantly increased relative to the normal values in healthy individuals. The blood microcirculation in nail bed capillaries is impaired as well. Furthermore, we demonstrate that the alterations of the parameters measured in vivo and in vitro for patients suffering from DM are interrelated. Good agreement between the results obtained with different techniques and their applicability for the diagnostics of microrheological properties of blood are demonstrated. Finally, we substantiate the possibility of using the reviewed methods in clinical practice as appropriate techniques for estimating blood microcirculation and microrheology disorders in DM.
- Добавил в систему: Лазарев Алексей Евгеньевич