|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
New Three-Finger Protein from Starfish Asteria rubens Shares Structure and Pharmacology with Human Brain Neuromodulator Lynx2статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Paramonov Alexander S., Shulepko Mikhail A., Makhonin Alexey M., Bychkov Maxim L., Kulbatskii Dmitrii S., Chernikov Andrey M., Myshkin Mikhail Yu, Shabelnikov Sergey V., Shenkarev Zakhar O., Kirpichnikov Mikhail P., Lyukmanova Ekaterina N.
- Журнал: Marine Drugs
- Том: 20
- Номер: 8
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 503
- DOI: 10.3390/md20080503
- Аннотация: Three-finger proteins (TFPs) are small proteins with characteristic three-finger β-structural fold stabilized by the system of conserved disulfide bonds. These proteins have been found in organ-isms from different taxonomic groups and perform various important regulatory functions or act as components of snake venoms. Recently, four TFPs (Lystars 1-4) with unknown function were identifyed in the coelomic fluid proteome of starfish A. rubens. Here we analyzed the genomes of A. rubens and A. planci starfish and predicted additional five and six TFPs, respectively. One of them, named Lystar5, is expressed in A. rubens coelomocytes and has sequence homology to the human brain neuromodulator Lynx2. The three-finger structure of Lystar5 close to the structure of Lynx2 was confirmed by NMR. Similar to Lynx2 Lystar5 negatively modulated α4β2 nicotinic ac-etylcholine receptors (nAChRs) expressed in X. laevis oocytes. Incubation with Lystar5 decreased the expression of acetylcholine esterase and α4 and α7 nAChR subunits in the hippocampal neu-rons. In summary, for the first time we reported modulator of the cholinergic system in starfish.
- Добавил в систему: Люкманова Екатерина Назымовна