Аннотация:Telomerase sustains telomere homeostasis and provides unlimited proliferative potential for unicellular eukaryotes, as well as stem, germ and cancer cells. The two core components of telomerase complex are telomerase RNA (TER) and telomerase reverse transcriptase (TERT). Functional and structural characterization of the telomerase is compromised by difficulties in obtaining suf- ficient amount of pure complex. Our group is developing a strategy of isolation of telomerase from thermotolerant yeast Hansenula polymorpha. Here we describe telomerase RNA of H. polymorpha (HpTER) tagged with an RNA hairpin, which is a binding target of the coat pro- tein of PP7 phage. Different sites of PP7 hairpin insertion were tested on the functionality of tagged HpTER in vivo and the abil- ity to recover HpTER from yeast extracts during affinity purifica- tion procedure.