An ultrasensitive bunge bedstraw herb type DNA machine for absolute quantification of mRNA in single cell

Chunhuan, X.; Zhao, J.; Chen, S.; Sakharov, I.Y.; Hu, S.; Zhao, S.

Авторы: Chunhuan Xu, Zhao Jingjin, Chen Shengyu, Sakharov Ivan Yu, Hu Shengqiang, Zhao Shulin
Журнал: Biosensors and Bioelectronics
Том: 217
Год издания: 2022
Издательство: Pergamon Press Ltd.
Местоположение издательства: United Kingdom
Номер статьи: 114702
DOI: 10.1016/j.bios.2022.114702
Аннотация: Messenger ribonucleic acids (mRNAs) comprise a class of small nucleic acids carrying genetic information, whichexhibit very important role in medical research and diagnosis. If only the mean mRNA expression levels of themRNA population are considered in medical research, important information linking mRNA expression andcellular function may be lost. Single-cell analysis provides valuable insights into studying its heterogeneity,signaling, and stochastic gene expression. In this study, a “bunge bedstraw herb”-type DNA machine based onDNAzyme catalyzing coupled clamping hybrid chain reaction (c-HCR) is presented. In the DNA machine, a bungebedstraw herb-type DNA structure was first formed by hybridizing a core junction scaffold cruciform probe to ahairpin probe that can trigger the c-HCR via a target molecule in four directions. This approach can reduce thedetection limit of mRNA to 5 × 10􀀀 15 M. Absolute quantification of survivin mRNA in individual cells wasachieved using the DNA machine on a microfluidic chip electrophoresis platform. The reported method representsan unprecedented single-cell analysis platform for single-cell biology studies.
Добавил в систему: Сахаров Иван Юрьевич

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