|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
Is the Fab-fragment from monoclonal rheumatoid IgM flexible?: A spin label dynamics studyстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 марта 2017 г.
- Авторы: Timofeev V.P., Tkachev Y.V., Novikov V.V., Alyoshkin V.A., Lapuk V.A.
- Журнал: Journal of Biomolecular Structure and Dynamics
- Том: 23
- Номер: 2
- Год издания: 2005
- Издательство: Adenine Press
- Местоположение издательства: United States
- Первая страница: 175
- Последняя страница: 181
- Аннотация: A comparative study of the Fab- and Fab-RF-fragments in the molecules of monoclonal IgM and IgM-RF, respectively, was performed by the spin label method. The spin label, 2,2,6,6-tetramethyl-4-dichloro-triazinylaminopiperidine-1-oxyl, was introduced into the peptide part of the protein. On the basis of the data on the temperature-viscosity dependences of the EPR spectral parameters of the resultant spin-labeled proteins, the rotational correlation time tau of the spin carrier was determined. It turned out that the reduced to normal conditions tau values for the molecules of the Fab- and Fab-RF-fragments were 21 +/- 2 and 11 +/- 1 ns, respectively. Analysis of the resultant data provides sufficient grounds for assuming that such a sharp decrease in the tau value for the molecule of the Fab-RF fragment is due to local flexibility of its globular structure, which, in turn, can determine the specific features of the IgMRF functioning as an autoantibody.
- Добавил в систему: Новиков Валентин Владимирович