Determination of mercury at the picogram per mililiter level using immobilized horseradish peroxidaseстатья
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Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
Аннотация:New test method and test device for the mercury (11) determination at the pg/mL level were developed based on the mercury inhbitory action on horseradish peroxidase immobilized on solid supports - in the cells of the polystyrene plate and on the chromatographic paper. The reactioiis of o-dianisidine, 3,3’,5,5’-tetramethylbenidine and o-phenylenediamine oxidation by hydrogen peroxide were used as the indicator reactions. The mercury inhibitory effect increased in the presence of thiourea. Under the
elucidated optimal conditions the calibration curves for the mercury determination showed a linear relationship between the peroxidaseinhibition degree and the mercury concentration in the range of 0,l-1000 pg/mL. The mercury detection limits were 0.1-10 pg/mL in dependence on the concrete indicator reaction. The analysis completed in 15 min. The proposed test device was applied to the mercury determination in underground waters of Moscow region. The mercury content obtained was with that obtained by atomic-fluorescent method with cold vapour.