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Electrochemical Detection of a Novel Pt(IV) Prodrug with the Metronidazole Axial Ligand in the Hypoxic Areaстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Spector Daniil V., Erofeev Alexander S., Gorelkin Petr V., Vaneev Alexander N., Akasov Roman A., Ul’yanovskiy Nikolay V., Nikitina Vita N., Semkina Alevtina S., Vlasova Kseniya Yu, Soldatov Mikhail A., Trigub Alexander L., Skvortsov Dmitry A., Finko Alexander V., Zyk Nikolay V., Sakharov Dmitry A., Majouga Alexander G., Beloglazkina Elena K., Krasnovskaya Olga O.
- Журнал: Inorganic Chemistry
- Том: 61
- Номер: 37
- Год издания: 2022
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 14705
- Последняя страница: 14717
- DOI: 10.1021/acs.inorgchem.2c02062
- Аннотация: We report herein a Pt(IV) prodrug with metronidazole in axial positions Pt-Mnz. The nitroaromatic axial ligand was conjugated with a cisplatin scaffold to irreversibly reduce under hypoxic conditions, thereby retaining the Pt(IV) prodrug in the area of hypoxia. X-ray near-edge adsorption spectroscopy (XANES) on dried drug-preincubated tumor cell samples revealed a gradual release of cisplatin from the Pt-Mnz prodrug instead of rapid intracellular degradation. The ability of the prodrug to penetrate into three-dimensional (3D) spheroid cellular cultures was evaluated by a novel electrochemical assay via a platinum-coated carbon nanoelectrode, capable of single-cell measurements. Using a unique technique of electrochemical measurements in single tumor spheroids, we were able to both detect the real-time response of the axial ligand to hypoxia and establish the depth of penetration of the drug into the tumor model.
- Добавил в систему: Красновская Ольга Олеговна