Spectroscopic and molecular dynamics characterization ofglycyrrhizin membrane-modifying activityстатьяИсследовательская статья
Статья опубликована в высокорейтинговом журнале
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 марта 2017 г.
Аннотация:Glycyrrhizic acid (GA) is a triterpene glycoside extracted from licorice root. Due to its amphiphilicityGA is capable of forming complexes with a variety of hydrophobic molecules, substantially increasingtheir solubility. GA can enhance the therapeutic effects of various drugs. It was hypothesized that theincreased bioavailability of the drug by GA is not only due to increased solubility, but also to enhancementof drug permeability through cell membranes. In this study the interaction of GA with POPC liposomesand model DOPC, POPC and DPPC bilayers was investigated by NMR with addition of shift reagents andMD simulations. This work helps to better understand the mechanism of enhanced drug bioavailability inthe presence of GA. NMR and MD reveal that GA does penetrate into the lipid bilayer. NMR shows that GAchanges the mobility of lipids. GA is predominantly located in the outer “half-layer” of the liposome andthat the middle of the hydrophobic tails is the preferred location. GA freely passes through the bilayersurface to the inner part bringing a few water molecules. Also both approaches indicate pore formation inthe presence of GA. The GA interaction with membranes is an additional aspect of the biological activityof GA-based drug delivery systems.