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Actin cytoskeleton and its regulatory proteins during regeneration in calcareous spongesтезисы доклада
- Авторы: Skorentseva K., Saidova A., Lavrov A.
- Сборник: 11th World Sponge Conference. Book of abstracts
- Тезисы
- Год издания: 2022
- Место издания: Naturalis Biodiversity Center Leiden, the Netherlands
- Первая страница: 225
- Последняя страница: 225
- Аннотация: The key step during regeneration in multicellular animals is epithelization, as it is crucially important to restore epithelial integrity. Two mechanisms provide epithelium restoration: the actomyosin cable “purse-string” contraction and the collective cell migration. However, these epithelization routes and their interaction with each other remain obscure. Therefore, it is important to study epithelization in basal multicellular animals such as sponges. Sponges are extant multicellular animals, they represent a sister group to other metazoans and have unique regeneration abilities. This study aimed to reveal actin cytoskeleton rearrangements and their possible regulation by special proteins during regenerative membrane formation in calcareous sponge Leucosolenia variabilis. To initiate regeneration, the oscular tube was cut transversely into rings 1-3 mm wide. The regeneration starts with the formation of a temporary structure, the regenerative membrane (RM), and leads to the formation of new body walls closing the wounds’ orifices. RM consists of three cell layers: exopinacoderm, endopinacoderm and the narrow mesohyl layer. It appears at ~12-24 hours after surgery and its formation relies on transformations of the preexisting cell layers and the contractile activity of the actin cable formed at the leading edge. The immunocytological studies allowed us to visualize and characterize the actin filaments in the cells of the intact body wall and the RM. We also quantitatively measured changes in cell morphological parameters (area, circularity and aspect ratio) that attendant cell layer transformations during regeneration. We as well found, annotated and predicted domain structure of small GTPases from Rho family and their effector proteins from the transcriptome and evaluated their mRNA expression (by RT-qPCR), These proteins are known to regulate actin filaments in eukaryotic cells and contribute to actin network rearrangement. The data obtained expand our knowledge about epithelial healing in basal multicellular animals, contributing to the field of evolutional and developmental biology.This study was supported by Russian Foundation for Basic Research grant no. 21-54-15006.
- Добавил в систему: Лавров Андрей Игоревич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | 11WSC_Scorentseva.pdf | 666,4 КБ | 22 октября 2022 [gonoduct] |