|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
Deeper insights into transcriptional features of cancer-associated fibroblasts: An integrated meta-analysis of single-cell and bulk RNA-sequencing dataстатья Исследовательская статья Электронная публикация
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Kazakova Anastasia N., Anufrieva Ksenia S., Ivanova Olga M., Shnaider Polina V., Malyants Irina K., Aleshikova Olga I., Slonov Andrey V., Ashrafyan Lev A., Babaeva Nataliya A., Eremeev Artem V., Boichenko Veronika S., Lukina Maria M., Lagarkova Maria A., Govorun Vadim M., Shender Victoria O., Arapidi Georgij P.
- Журнал: Frontiers in Cell and Developmental Biology
- Том: 10
- Год издания: 2022
- Издательство: Frontiers Media S.A.
- Местоположение издательства: Lausanne, Switzerland
- Номер статьи: 825014
- DOI: 10.3389/fcell.2022.825014
- Аннотация: Cancer-associated fibroblasts (CAFs) have long been known as one of the most important players in tumor initiation and progression. Even so, there is an incomplete understanding of the identification of CAFs among tumor microenvironment cells as the list of CAF marker genes varies greatly in the literature, therefore it is imperative to find a better way to identify reliable markers of CAFs. To this end, we summarized a large number of single-cell RNA-sequencing data of multiple tumor types and corresponding normal tissues. As a result, for 9 different types of cancer, we identified CAF-specific gene expression signatures and found 10 protein markers that showed strongly positive staining of tumor stroma according to the analysis of IHC images from the Human Protein Atlas database. Our results give an insight into selecting the most appropriate combination of cancer-associated fibroblast markers. Furthermore, comparison of different approaches for studying differences between cancer-associated and normal fibroblasts (NFs) illustrates the superiority of transcriptome analysis of fibroblasts obtained from fresh tissue samples. Using single-cell RNA sequencing data, we identified common differences in gene expression patterns between normal and cancer-associated fibroblasts, which do not depend on the type of tumor.
- Добавил в систему: Мальянц Ирина Кареновна