Spectroscopy Study of Albumin Interaction with Negatively Charged Liposome Membranes: Mutual Structural Effects of the Protein and the Bilayers

Tretiakova, D.; Kobanenko, M.; Le-Deygen, I.; Boldyrev, I.; Kudryashova, E.; Onishchenko, N.; Vodovozova, E.

Авторы: Tretiakova Daria, Kobanenko Maria, Le-Deygen Irina, Boldyrev Ivan, Kudryashova Elena, Onishchenko Natalia, Vodovozova Elena
Журнал: Membranes
Том: 12
Номер: 11
Год издания: 2022
Издательство: MDPI Publishing
Местоположение издательства: Basel, Switzerland, Switzerland
Первая страница: 1031
DOI: 10.3390/membranes12111031
Аннотация: Liposomes as drug carriers are usually injected into the systemic circulation where they are instantly exposed to plasma proteins. Liposome–protein interactions can affect both the stability of liposomes and the conformation of the associated protein leading to the altered biodistribution of the carrier. In this work, mutual effects of albumin and liposomal membrane in the course of the protein’s adsorption were examined in terms of quantity of bound protein, its structure, liposome membrane permeability, and changes in physicochemical characteristics of the liposomes. Fluorescence spectroscopy methods and Fourier transform infrared spectroscopy (ATR-FTIR), which provides information about specific groups in lipids involved in interaction with the protein, were used to monitor adsorption of albumin with liposomes based on egg phosphatidylcholine with various additives of negatively charged lipidic components, such as phosphatidylinositol, ganglioside GM1, or the acidic lipopeptide. Less than a dozen of the protein molecules were tightly bound to a liposome independently of bilayer composition, yet they had a detectable impact on the bilayer. Albumin conformational changes during adsorption were partially related to bilayer microhydrophobicity. Ganglioside GM1 showed preferable features for evading undesirable structural changes
Добавил в систему: Ле-Дейген Ирина Михайловна

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Spectroscopy Study of Albumin Interaction with Negatively Charged Liposome Membranes: Mutual Structural Effects of the Protein and the Bilayersстатья Исследовательская статья

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