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Abnormal left-right organizer and laterality defects in Xenopus embryos after formin inhibitor SMIFH2 treatmentстатья Исследовательская статья
- Авторы: Petri Natalia, Nordbrink Rhea, Tsikolia Nikoloz, Kremnyov Stanislav
- Журнал: PLoS ONE
- Том: 17
- Номер: 11
- Год издания: 2022
- Издательство: Public Library of Science
- Местоположение издательства: United States
- Первая страница: e0275164
- DOI: 10.1371/journal.pone.0275164
- Аннотация: Left-right symmetry breaking in most studied vertebrates makes use of so-called leftwardflow, a mechanism which was studied in detail especially in mouse and Xenopus laevisembryos and is based on rotation of monocilia on specialized epithelial surface designatedas left-right organizer or laterality coordinator. However, it has been argued that prior toemergence of leftward flow an additional mechanism operates during early cleavage stagesin Xenopus embryo which is based on cytoskeletal processes. Evidence in favour of thisearly mechanism was supported by left-right abnormalities after chemical inhibition of cytoskeletalprotein formin. Here we analyzed temporal dimension of this effect in detail andfound that reported abnormalities arise only after treatment at gastrula-neurula stages, i.e.just prior to and during the operation of left-right organizer. Moreover, molecular and morphologicalanalysis of the left-right organizer reveals its abnormal development. Our resultsstrongly indicate that left-right abnormalities reported after formin inhibition cannot serve assupport of models based on early symmetry breaking event in Xenopus embryo
- Добавил в систему: Кремнёв Станислав Валерьевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Текст статьи | journal.pone.0275164.pdf | 2,8 МБ | 10 ноября 2022 [Kremnyov] |