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Oak bark (Quercus sp. cortex) protects plants through the inhibition of quorum sensing mediated virulence of Pectobacterium carotovorumстатья Исследовательская статья
- Авторы: Vasilchenko Alexey S., Poshvina Darya V., Sidorov Roman Yu, Iashnikov Aleksandr V., Rogozhin Eugene A., Vasilchenko Anastasia V.
- Журнал: World Journal of Microbiology and Biotechnology
- Том: 38
- Номер: 11
- Год издания: 2022
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 184
- DOI: 10.1007/s11274-022-03366-6
- Аннотация: Bacterial intercellular communication mediated by small diffusible molecules, known as quorum sensing (QS), is a common mechanism for regulating bacterial colonisation strategies and survival. Influence on QS by plant-derived molecules is proposed as a strategy for combating phytopathogens by modulating their virulence. This work builds upon other studies that have revealed plant-derived QS inhibitors extracted from oak bark (Quercus sp.). It was found that co-incubation of Pectobacterium carotovorum VKM-B-1247 with oak bark extract (OBE) reduced the production of acyl-HSL. This was accompanied by a dose-dependent decrease in the bacterial cellulolytic and protease activity. At the transcriptomic level, the OBE treatment suppressed the main QS-related genes expR/expI. Potato tubers pre-treated with OBE showed resistance to a manifestation of soft-rot symptoms. Analysis of the component composition of the OBE identified several biologically active molecules, such as n-hexadecanoic acid, 2,6-di-tert-butyl-4-methylphenol, butylated hydroxytoluene (BHT), gamma-sitosterol, lupeol, and others. Molecular docking of the binding energy between identified molecules and homology models of LuxR-LuxI type proteins allow to identify potential inhibitors. Collectively, obtained results figure out great potential of widely distributed oak-derived plant material for bacterial control during storage of potato.
- Добавил в систему: Васильченко Анастасия Валерьевна