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Properties of intracytoplasmic A particles isolated from oncornavirus-producing human cells. PMID: 4138440 PMCID: PMC355600 DOI: 10.1128/JVI.14.4.924-933.1974статья
- Авторы: Bukrinskaya AG, GG Miller, EN Lebedeva, LV Agaphonova, NN Masurenko, KV Ilyin
- Журнал: Journal of Virology
- Том: 14
- Номер: 4
- Год издания: 1974
- Издательство: American Society for Microbiology
- Местоположение издательства: United States
- Первая страница: 924
- Последняя страница: 933
- Аннотация: A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradients suggesting that they contained 8% RNA. Individual A particles sedimented at 200 to 250S in velocity sucrose gradients, but their significant part was found aggregated and sedimented at more than 300S. They were resistant to RNase digestion. A particles possessed polymerase activity which was preferentially activated by Mn(2+) rather than by Mg(2+), the RNA template being 60S RNA. Cross-hybridization with two DNA products and immunoassay showed that A particles and Mason-Pfizer-like oncornavirus produced by the same cells contained neither homological RNA sequences nor common antigens, suggesting that A particles are not intracellular precursors of Mason-Pfizer-like oncornavirus but represent an independent oncornavirus. Hybridization of A particle RNA with excess of cellular DNA revealed about 20 proviral copies per HEp-2 cell genome and no proviral copies in human embryo and placenta cell genomes.
- Добавил в систему: Мазуренко Наталья Николаевна