Preparation of Catalase Cross-Linked Aggregates Based on Vaterite Matrix

Tagirova, M.A.; Eremeev, N.L.; Balabushevich, N.G.; Volodkin, D.V.; Klyachko, N.L.

Авторы: Tagirova M.A., Eremeev N.L., Balabushevich N.G., Volodkin D.V., Klyachko N.L.
Журнал: Applied Biochemistry and Microbiology
Том: 58
Номер: 8
Год издания: 2022
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 923
Последняя страница: 931
DOI: 10.1134/S0003683822080075
Аннотация: M. A. Tagirova, N. L. Eremeev, N. G. Balabushevich, D. V. Volodkin, N. L. Klyachko (2022). Preparation of Catalase Cross-Linked Aggregates Based on Vaterite Matrix. Applied Biochemistry and Microbiology, 2022, Vol. 58, No. 8, P. 923–931. Published Nov. 30, 2022. DOI: 10.1134/S0003683822080075IF 1.065 Pleiades Publishing, Inc.ISSN 0003-6838 Applied Biochemistry and Microbiology, 2022, Vol. 58, No. 8, P. 923–931.Russian Text © The Author(s), 00000, published in Biotekhnologiya, 2021, Vol. 37, No. 4, pp. 51–59.A new technique has been developed for the synthesis of cross-linked catalase aggregates by treatment of the enzyme incorporated into the pores of vaterite microspheres with glutaraldehyde and subsequent dissolving of the inorganic matrix. The resulting aggregates have a spherical shape, a narrow particle size distribution, and a high specific activity. The number and enzyme activity of the cross-linked aggregates strongly depends on the rate of the matrix dissolution: mild conditions of dissolution made it possible to increase the number of formed protein particles, whose residual catalase specific activity was only 2 times less than that of the native enzyme. The storage stability of the cross-linked aggregates is comparable to that of the native enzyme of the same concentration.
Добавил в систему: Клячко Наталья Львовна

	ИСТИНА	Войти в систему Регистрация
	ИСТИНА ИНХС РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИСТИНА ИНХС РАН

Preparation of Catalase Cross-Linked Aggregates Based on Vaterite Matrixстатья

Прикрепленные файлы