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Methodological aspects of intraarterial catheter placement for stem cell transplantation in rats with experimental stroke / 14th World Stroke Congress, Singapore, 26-29 October 2022статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Gubskiy I., Namestnikova D., Cherkashova E., Lelyuk V., Gubskiy L., Yarygin K.
- Журнал: International Journal of Stroke
- Том: 17
- Номер: 3_suppl
- Год издания: 2022
- Издательство: Blackwell
- Местоположение издательства: United Kingdom
- Первая страница: 204
- Последняя страница: 204
- DOI: 10.1177/17474930221125973
- Аннотация: Background and Aims: Stem cell-based therapy of stroke is a promising approach, offering hope for preservation of damage brain tissue. There are various ways of cells administration, among which intra-arterial delivery has shown to be one of the most effective and challenging. Technical inaccuracies with intra-arterial transplantation can lead to the development of serious complications, such as thromboembolism. In this study we examine several methodological aspects of intra-arterial catheter placement in rats with experimantal stroke model, including catheter position, maintenance of blood flow around it and possible development of vasospasm after surgery.Methods: Wistar rats 250-300g were subjected to transient 90min MCAO and after 24h microcatheter was inserted via the ECA into the lumen of the ICA in the direction of blood flow (n=4) or into the CCA (n=44) in the opposite direction of blood flow. After catheter placement experimental animals were transferred into MR-scaner for visualization of the catheter and cerebral arteries.Results: In the group with the location of the catherer in the ICA in most animals there was no blood flow in the CCA and proximal part of the ICA. Vasospams was observed in both experimental groups. For the group with the location of the catherer in the CCA the following ratio of vasospasm was determined: 41% weak, 41% local and 18% strong.Conclusions: Placement of the intra-arterial catheter into the CCA reduces the chance of brachiocephalic arteries occlusion and ensures cells administration with maintenance of blood flow. During transplantation, it is worth considering the possibility of angiospasm as a result of endovascular interventions.
- Добавил в систему: Лелюк Владимир Геннадьевич