Adsorption of human blood plasma on nanodiamond and its influence on activated partial thromboplastin timeстатья
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Дата последнего поиска статьи во внешних источниках: 7 декабря 2013 г.
Аннотация:Ever increasing use of engineered nanodiamond (ND) into the human blood for various biomedical studies and
applications has increased the demand to thoroughly understand the interaction of NDswith blood and its effect
on blood coagulation. Here, we report on the study of adsorption of human blood plasma on various sized
carboxylated nanodiamonds (cNDs) using UV/visible spectroscopy and Fourier transform infrared spectroscopy
(FTIR). The adsorption of human blood plasma on 5 nm and 100 nm sized cNDs is confirmed from UV/visible
spectra. FTIR shows minor change in the shape of amide I absorption peak (1600–1700 cm−1) indicating that
the protein secondary structure remains unaffected for human blood plasma. The influence of cNDs on the
blood coagulation has been estimated using Activated Partial Thromboplastin Time (APTT) test. The APTT test
is one of the standard tests used to investigate the efficacy of the intrinsic pathways of coagulation. The APTT
test results indicate that 5 and 100 nm cNDs with various concentrations (10–500 μg/ml) do not show delay
in time when coagulation was initiated through the intrinsic pathway.