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New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cellsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 апреля 2017 г.
- Авторы: Vedyaykin Alexey D., Vishnyakov Innokentii E., Polinovskaya Vasilisa S., Khodorkovskii Mikhail A., Sabantsev Anton V.
- Журнал: MicrobiologyOpen
- Том: 5
- Номер: 3
- Год издания: 2016
- Издательство: Wiley & Sons
- Местоположение издательства: Chichester, Sussex, UK, England
- Первая страница: 378
- Последняя страница: 386
- DOI: 10.1002/mbo3.336
- Аннотация: FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from consensus on this question. We utilized single-molecule localization microscopy (SMLM) in combination with immunofluorescence staining to visualize FtsZ in Esherichia coli fixed cells that were grown under slow and fast growth conditions. This approach allowed us to obtain images of FtsZ structures at different stages of cell division and accurately measure Z-ring dimensions. Analysis of these images demonstrated that Z-ring thickness increases during constriction, starting at about 70nm at the beginning of division and increasing by approximately 25% half-way through constriction.
- Добавил в систему: Вишняков Иннокентий Евгеньевич