|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
[ATM/ATR signaling pathway activation in human embryonic stem cells after DNA damage]статья
Дата последнего поиска статьи во внешних источниках: 21 августа 2017 г.
- Авторы: Suvorova I.I., Kozhukharova I.V., Nikol’ski? N.N., Pospelov V.A.
- Журнал: Tsitologiya
- Том: 55
- Номер: 12
- Год издания: 2013
- Издательство: Nauka Publishers
- Местоположение издательства: United States
- Первая страница: 841
- Последняя страница: 851
- Аннотация: Embryonic stem cells (ESCs) are the progenitors of all adult cells so any disruption in their genome can have disastrous consequences for the developing organism. ESCs are characterized by a high proliferation activity and do not undergo checkpoints upon DNA-damage executing only G2/M delay after DNA damage. ATM and ATR kinase are key sensors of DNA double strands breaks and activate downstream signaling pathways involving checkpoints, DNA repair and apoptosis. We estimated ATM/ATR signaling pathway activation in human ESCs and have revealed that irradiation induced ATM, ATR Chk2 phosphorylation, γH2AX foci formation and their co-localization with 53BP1 and Rad51 proteins. Interestingly, human ESCs display non-induced yH2AX foci co-localized with Rad51 and marking DNA single-strand breaks. Next we have revealed the substantial contribution of ATM, Chk1 and Chk2 kinases to G2/M block after irradiation of human ESCs and ATM-dependent activation (phosphorylation) of p53. However p53 activation and subsequent induction of p21 gene expression after DNA damage do not result in p21 protein accumulation due to proteasomal degradation.
- Добавил в систему: Суворова Ирина Игоревна