Аннотация:The aim of this study was to determine the influence of high-intensity training underextreme conditions (T = 40 ◦C) on the metabolism and immunological reactions of athletes. Maletriathletes (n = 11) with a high level of sports training performed load testing to failure (17 ± 2.7 min)and maximum oxygen consumption (64.1 ± 6.4 mL/min/kg). Blood plasma samples were collectedbefore and immediately after exercise. Mass spectrometric metabolomic analysis identified 30 metabolites and 6 hormones in the plasma, of which 21 and 4 changed after exercise, respectively. Changesin the intermediate products of tricarboxylic and amino acids were observed (FC > 1.5) after exercise.The obtained data can be associated with the effect of physical activity on metabolism in athletes.Therefore, constant monitoring of the biochemical parameters of athletes can help coaches identifyindividual shortcomings in a timely manner and track changes, especially as the volume of trainingincreases. In addition, it was revealed that the immunological reaction (manifestation of a hyperactivereaction to food components) is personalized in nature. Therefore, it is important for coaches andsports doctors to analyze and control the eating behavior of athletes to identify food intolerances orfood allergies in a timely manner and develop an individual elimination diet.