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Immunofluorescence Analysis of Erythrocyte Membranes of Cervical Cancer Patientsстатья
- Авторы: Mamaeva Sargylana N., Alekseev Vladislav A., Nikolaeva Nadezhda A., Krylova Tatyana A., Gabysheva1 Aya A., Pavlov Alexandr N., Kononova Irina V., Maksimov Georgy V.
- Журнал: International Journal of Biomedicine
- Том: 13
- Номер: 1
- Год издания: 2023
- Первая страница: 69
- Последняя страница: 72
- DOI: 10.21103/Article13(1)_OA8
- Аннотация: Currently, cervical cancer (CC) is one of the most common oncological diseases. In this regard, it is necessary to developnew research methods for a more detailed study of the occurrence and development of the disease at the molecular and cellularlevels, as well as to improve the effectiveness of treatment and form a deeper understanding of the causes of relapses. The aim ofthis work was to study nanoparticles localized on the erythrocyte membrane—presumably HPV 16, 18, before and after radiationtherapy, in patients with CC.To study the surface of red blood cells by the SEM method, venous blood samples from 17 patients with a confirmeddiagnosis of CC were prepared in thin layers evenly applied to a dry, fat-free glass slide, which was dried at room temperature.To detect nanoparticles on the surface of erythrocytes by the immunofluorescence assay, we developed a special protocol forpreparing erythrocyte masses from patients diagnosed with CC. As a result of using a new method of sample preparation forimmunofluorescence assay and using SEM, the hypothesis of the viral nature of nanoparticles localized on the surface of theblood erythrocytes of patients with CC was confirmed: Particles of HPV 16 and 18 are located on the cytoplasmic membrane oferythrocytes. Studies suggest that viruses attach to the erythrocyte membrane, which seems to influence the development of CC,its recurrence, and metastasis.(International Journal of Biomedicine. 2023;13(1):69-72.)
- Добавил в систему: Максимов Георгий Владимирович