pilE G-QuadruplexIs recognized and preferentially bound but not processed by the MutL endonuclease from Neisseria gonorrhoeae mismatch repair pathway

Savitskaya, V.Y.; Strekalovskikh, V.V.; Snyga, V.G.; Monakhova, M.V.; Arutyunyan, A.M.; Dolinnaya, N.G.; Kubareva, E.A.

Статья опубликована в высокорейтинговом журнале

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Авторы: Savitskaya V.Y., Strekalovskikh V.V., Snyga V.G., Monakhova M.V., Arutyunyan A.M., Dolinnaya N.G., Kubareva E.A.
Журнал: International Journal of Molecular Sciences
Том: 24
Номер: 7
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 6167
DOI: 10.3390/ijms24076167
Аннотация: The human pathogen Neisseria gonorrhoeae uses a homologous recombination to undergo antigenic variation and avoid an immune response. The surface protein pilin (PilE) is one of thetargets for antigenic variation that can be regulated by N. gonorrhoeae mismatch repair (MMR) and a G-quadruplex (G4) located upstream of the pilE promoter. Using bioinformatics tools, we found a correlation between pilE variability and deletion of DNA regions encoding ngMutS or ngMutLproteins, the main participants in N. gonorrhoeae methyl-independent MMR. To understand whetherthe G4 structure could affect the ngMutL-mediated regulation of pilin antigenic variation, we designed several synthetic pilE G4-containing oligonucleotides, differing in length, and related DNA duplexes.Using CD measurements and biochemical approaches, we have showed that (i) ngMutL preferentiallybinds to pilE G4 compared to DNA duplex, although the latter is a cognate substrate for ngMutLendonuclease, (ii) protein binding affinity decreases with shortening of quadruplex-containing and duplex ligands, (iii) the G4 structure inhibits ngMutL-induced DNA nicking and modulates cleavage positions; the enzyme does not cleave DNA within G4, but is able to bypass this noncanonicalstructure. Thus, pilE G4 may regulate the efficiency of pilin antigenic variation by quadruplex binding to ngMutL and suppression of homologous recombination.
Добавил в систему: Кубарева Елена Александровна

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