Poly-(lactic-co-glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Aspergillus Section Nigri

Orekhova, A.; Palocci, C.; Chronopoulou, L.; De Angelis, G.; Badiali, C.; Petruccelli, V.; Simonetti, G.

Авторы: Orekhova A., Palocci C., Chronopoulou L., De Angelis G., Badiali C., Petruccelli V., Simonetti G.
Журнал: Molecules
Том: 27
Номер: 7
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
DOI: 10.3390/molecules27175424
Аннотация: first_pagesettingsOrder Article ReprintsOpen AccessArticlePoly-(lactic-co-glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Aspergillus Section Nigriby Anastasia Orekhova 1,†,Cleofe Palocci 2,3,†,Laura Chronopoulou 2ORCID,Giulia De Angelis 4,Camilla Badiali 4,Valerio Petruccelli 4,Simone D’Angeli 4ORCID,Gabriella Pasqua 4 andGiovanna Simonetti 4,*ORCID1Department of Public Health and Infectious Diseases, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy2Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy3Research Center for Applied Sciences to the Safeguard of Environment and Cultural Heritage (CIABC), P.le A. Moro 5, 00185 Rome, Italy4Department of Environmental Biology, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy*Author to whom correspondence should be addressed.†These authors contributed equally to this work.Molecules 2022, 27(17), 5424; https://doi.org/10.3390/molecules27175424Received: 30 July 2022 / Revised: 19 August 2022 / Accepted: 21 August 2022 / Published: 25 August 2022(This article belongs to the Special Issue Advances in Anti-infective Drug Discovery)Download Browse Figures Review Reports Versions NotesAbstractPoly-(lactic-co-glycolic) acid (PLGA) is a biodegradable, biosafe, and biocompatible copolymer. The Aspergillus section Nigri causes otomycosis localized in the external auditory canal. In this research, Aspergillus brasiliensis, a species belonging to the Nigri section, was tested. Coumarin 6 and pterostilbene loaded in poly-(lactic-co-glycolic) acid nanoparticles (PLGA-coumarin6-NPs and PLGA-PTB-NPs) were tested for fungal cell uptake and antifungal ability against A. brasiliensis biofilm, respectively. Moreover, the activity of PLGA-PTB-NPs in inhibiting the A. brasiliensis infection was tested using Galleria mellonella larvae. The results showed a fluorescence signal, after 50 nm PLGA-coumarin6-NPs treatment, inside A. brasiliensis hyphae and along the entire thickness of the biofilm matrix, which was indicative of an efficient NP uptake. Regarding antifungal activity, a reduction in A. brasiliensis biofilm formation and mature biofilm with PLGA-PTB-NPs has been demonstrated. Moreover, in vivo experiments showed a significant reduction in mortality of infected larvae after injection of PLGA-PTB-NPs compared to free PTB at the same concentration. In conclusion, the PLGA-NPs system can increase the bioavailability of PTB in Aspergillus section Nigri biofilm by overcoming the biofilm matrix barrier and delivering PTB to fungal cells.
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Poly-(lactic-co-glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Aspergillus Section Nigriстатья

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