Аннотация:Neuroinflammation plays an important role in epileptogenesis, however, most studies are performed usingpharmacological models of epilepsy, while there are only few data available for non-invasive, including genetic, models.The levels of a number of pro-inflammatory cytokines were examined in the Krushinsky–Molodkina (KM) rat strain withhigh audiogenic epilepsy (AE) proneness (intense tonic seizure fit in response to loud sound) and in the control strain “0”(not predisposed to AE) using multiplex immunofluorescence magnetic assay (MILLIPLEX map Kit). Cytokine levels weredetermined in the dorsal striatum tissue and in the brain stem. Background levels of IL-1β, IL-6, and TNF-α in the dorsalstriatum of the KM rats were significantly lower than in the rats “0” (by 32.31, 27.84, and 38.87%, respectively, p < 0.05,0.05, and 0.01), whereas no inter-strain differences in the levels of these metabolites were detected in the brain stem in the“background” state. Four hours after sound exposure, the TNF-α level in the dorsal striatum of the KM rats was significantly lower (by 38.34%, p < 0.01) than in the “0” rats. In the KM rats, the dorsal striatal levels of IL-1β and IL-6 weresignificantly higher after the sound exposure and subsequent seizure fit, compared to the background (35.29 and 50.21%increase, p < 0.05, 0.01, respectively). In the background state the IL-2 level in the KM rats was not detected, whereas afteraudiogenic seizures its level was 14.01 pg/ml (significant difference, p < 0.01). In the KM rats the brain stem levels of IL-1βand TNF-α after audiogenic seizures were significantly lower than in the background (13.23 and 23.44% decrease, respectively, p < 0.05). In the rats of the “0” strain, the levels of cytokines in the dorsal striatum after the action of sound (whichdid not induce AE seizures) were not different from those of the background, while in the brain stem of the “0” strain thelevels of IL-1β were lower than in the background (40.28%, p < 0.01). Thus, the differences between the background levelsof cytokines and those after the action of sound were different in the rats with different proneness to AE. These data suggestinvolvement of the analyzed cytokines in pathophysiology of the seizure state, namely in AE seizures.
