Characterization and Genomic Analysis of the Naphthalene-Degrading Delftia tsuruhatensis ULwDis3 Isolated from Seawater

Sazonova, O.I.; Ivanova, A.A.; Delegan, Y.A.; Streletskii, R.A.; Vershinina, D.D.; Sokolov, S.L.; Vetrova, A.A.

Авторы: Sazonova Olesya I., Ivanova Anastasia A., Delegan Yanina A., Streletskii Rostislav A., Vershinina Diana D., Sokolov Sergei L., Vetrova Anna A.
Журнал: Microorganisms
Том: 11
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 1
Последняя страница: 14
Номер статьи: 1092
DOI: 10.3390/microorganisms11041092
Аннотация: Strains of the genus Delftia are poorly studied microorganisms. In this work, the completegenome of the naphthalene-degrading Delftia tsuruhatensis strain ULwDis3 isolated from seawater ofthe Gulf of Finland of the Baltic Sea was assembled. For the first time, genes encoding naphthalenecleavage pathways via salicylate and gentisate were identified in a strain of the genus Delftia. Thegenes are part of one operon (nag genes). Three open reading frames (ORFs) were found in thegenome of D. tsuruhatensis strain ULwDis3 that encode gentisate 1.2-dioxygenase. One of the ORFs ispart of the nag operon. The physiological and biochemical characteristics of the strain ULwDis3 whencultured in mineral medium with naphthalene as the sole source of carbon and energy were alsostudied. It was found that after 22 h of growth, the strain stopped consuming naphthalene, and at thesame time, naphthalene 1.2-dioxygenase and salicylate 5-hydroxylase activities were not detected.Later, a decrease in the number of living cells and the death of the culture were observed. Gentisate1.2-dioxygenase activity was detected from the time of gentisate formation until culture death.
Добавил в систему: Стрелецкий Ростислав Александрович

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Characterization and Genomic Analysis of the Naphthalene-Degrading Delftia tsuruhatensis ULwDis3 Isolated from Seawaterстатья

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