Activation of Bone Marrow Multipotent Stromal Cells by Laser and EHF Radiation and Their Combined Impactsстатья
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 5 июня 2017 г.
Аннотация:bone marrow multipotent stromal cells (MSCs) in “normal” and “suppressed” states in vitro, as well as the ability of these factors to
influence the content of MSCs in the bone marrow in vivo and in vitro.
Materials and Methods. Laser radiation of low and moderate intensity, acoustic pulses generated by laser radiation in biological tissue,
and EHF radiation have been used for mono and combined (applied for the first time) impacts on MSCs in vivo and in vitro. Short-term
fragmentary laser heating of rat shins in vivo has been used to stimulate the colony-forming efficiency of MSCs. Stimulation of proliferative
activity and MSCs content were studied on the strains derived from human bone marrow, rabbits, guinea pigs and rats.
Irradiation of MSCs strains was performed in the “normal” state, as well as in the “suppressed” strains with the decreased proliferative
activity induced by the reduction of fetal serum concentration in the nutrient medium of the cultivated cells. Exposure doses were varied by
altering the power and time of irradiation.
Results. A twofold increase of colony number was observed when the bone marrow was heated by a laser irradiation, and a marked
stimulation of colony-forming efficiency exceeding the reference values by 85% under EHF irradiation of bone marrow suspension with the
dose of 8 J/cm2 was also noted.
The effect of physical factors greatly depends on the MSCs state: there is a significant enhancement of proliferative activity of the
cells being in the “suppressed” state. Acoustic pulses of laser-induced hydrodynamics cause a statistically significant (p<0.01) increase of
proliferative activity of human MSCs (by 80% relative to the control). The proliferative activity of human MSCs was not enhanced under
combined impacts compared to the exposure to mono acoustic pulses of laser-induced hydrodynamics.
Conclusion. The studied physical effects in vivo and in vitro increase the content of MSCs in the initial bone marrow, as well as their
proliferative activity in the process of MSCs strains development in vitro. Application of these techniques in clinic will make it possible to
obtain the necessary cell number at earlier passages for autologous MSCs transplantation preventing thereby chromosomal aberrations in
MSCs cultures.