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Ca2+-myristoyl switch in the neuronal calcium sensor recoverin requires different functions of Ca2+-binding sitesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Senin I.I., Fischer T., Komolov K.E., Zinchenko D.V., Philippov P.P., Koch K.W.
- Журнал: Journal of Biological Chemistry
- Том: 277
- Номер: 52
- Год издания: 2002
- Издательство: American Society for Biochemistry and Molecular Biology Inc.
- Местоположение издательства: United States
- Первая страница: 50365
- Последняя страница: 50372
- DOI: 10.1074/jbc.M204338200
- Аннотация: Recoverin is an EF-hand Ca2+-binding protein that is suggested to control the activity of the G-protein-coupled receptor kinase GRK-1 or rhodopsin kinase in a Ca2+-dependent manner. It undergoes a Ca2+-myristoyl switch when Ca2+ binds to EF-hand 2 and 3. We investigated the mechanism of this switch by the use of point mutations in EF-hand 2 (E85Q) and 3 (E121Q) that impair their Ca2+ binding. EF-hand 2 and 3 display different properties and serve different functions. Binding of Ca2+ to recoverin is a sequential process, wherein EF-hand 3 is occupied first followed by the filling of EF-hand 2. After EF-hand 3 bound Ca2+, the subsequent filling of EF-hand 2 triggers the exposition of the myristoyl group and in turn binding of recoverin to membranes. In addition, EF-hand 2 controls the mean residence time of recoverin at membranes by decreasing the dissociation rate of recoverin from membranes by 10-fold. We discuss this mechanism as one critical step for inhibition of rhodopsin kinase by recoverin.
- Добавил в систему: Филиппов Павел Павлович