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Delivery Properties of Polycations Based on 2-(N,N-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymersстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Loginova T.P., Khotina I.A., Kabachii Y.A., Kochev S.Y., Abramov V.M., Khlebnikov V.S., Kulikova N.L., Mezhuev Y.O.
- Журнал: Polymers
- Том: 15
- Номер: 14
- Год издания: 2023
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 3036
- Последняя страница: 3040
- Номер статьи: 2408993
- DOI: 10.3390/polym15143036
- Аннотация: Cationic copolymers based on 2-(N,N-dimethylamino)ethyl methacrylate and polyethylene glycol monomethyl ether (pDMAEMA-co-PEO) with different molecular weights have been synthesized. Their physicochemical properties were studied by NMR spectroscopy, sedimenta-tion, and potentiometric titration. According to the data of potentiometric titration for the syn-thesized pegylated cationic copolymers, the apparent dissociation constants were determined in the pH range from 4.5 to 8.5. The physicochemical properties of interpolyelectrolyte complexes of these polycations with circular DNA (IPEC DNA) were also studied by dynamic light scatter-ing, electrophoretic mobility, and TEM methods. It has been established that the diameter and electrokinetic potential (ζ- potential) of interpolyelectrolyte complexes can be varied over a wide range (from 200 nm to 1.5 μm and from −25 mV to +30 mV) by changing the ratio of oppo-sitely charged ionizable groups in pegylated cationic copolymers and DNA, as well as by regu-lating medium pH. The resistance of the IPEC DNA/polycation complex to the action of nucleas-es was studied by electrophoresis in agarose gel; the cytotoxic effect of the polymers in vitro, and the efficiency of penetration (transfection) of IPEC DNA with PDMAE-MA-co-PEO-polycations into eukaryotic cells of a cell line derived from human embryonic kid-neys HEK 293 in vitro.
- Добавил в систему: Хотина Ирина Анатольевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Akt_ekspertizyi.docx | Akt_ekspertizyi.docx | 106,8 КБ | 13 июля 2023 [ikhotina] | |
| 2. | polymers-2408993_Proof.docx | polymers-2408993_Proof.docx | 1,4 МБ | 13 июля 2023 [ikhotina] |