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A Rapid Method for the Detection of Metabolite of Sulfur Mustard 1,1’-Sulfonylbis[2-S-(N-Acetylcysteinyl)Ethane] in Biofluids via LC-MS/MSтезисы доклада
- Авторы: Родин И.А., Браун А.В., Шпигун О.А., Ананьева И.А., Рыбальченко И.В.
- Сборник: In book of abstract 36th International Symposium on High-Performance Liquid Chromatography and Related Techniques
- Тезисы
- Год издания: 2011
- Место издания: Будапешт, Венгрия
- Первая страница: 75
- Аннотация: Sulfur mustard is a widely known chemical warfare agent. In Russia and former USSR republics heavy stocks of ammunition containing this agent are stored. Industrial destruction of these dangerous objects has been taking place recently. Control of environmental and human safety in regions located near factories is an important analytical task. Another reason of creating analytical technique for the determination of chemical warfare agents is a threat of application of sulfur mustard in acts of terror. After human intoxication by sulfur mustard it metabolizes producing various metabolites. Some of them are not stable, but one – 1,1’-sulfonyl-bys-[2-S-(N-acetylcysteinyl)etan is stable enough to be found in plasma for several days. This compound is the most suitable for being used as a sulphur mustard intoxication marker. In present work LC-MS/MS technique was used for the determination of 1,1’-sulfonylbys-[ 2-S-(N-acetylcysteinyl)etan in plasma samples. The procedure of sample pretreatment included steps of plasma deproteinization with 10% water solution of trichloracetic acid, and solid phase extraction to clean the sample. HPLC separation was carried out on a reversed-phase column (Synergi RP Hydro (150x2.1 mm) using water-acetonitrile as a mobile phase (pH 5.4). The detection in a negative electrospray ionization mode provided good analytical signal. Deprotonised molecular ion (m/z = 443, as a parent ion) and its fragments (m/z = 163.5 and 127) were used for the detection in MRM. Limit of detection was about 0,1 ng/ml. Validation of technique was carried out using plasma of rats, intoxicated by sulfur mustard (about ½ LD 50). 1,1’-sulfonyl-bys-[2-S-(N-acetylcysteinyl)etan was found in rat plasma and urea up to 144 hours after intoxication.
- Добавил в систему: Ставрианиди Андрей Николаевич