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Reactive oxygen species are required for human mesenchymal stem cells to initiate proliferation after the quiescence exit. Oxid Med Cell Longevстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 октября 2017 г.
- Авторы: Lyublinskaya O.G., Borisov Ya G., Pugovkina N.A., Smirnova I.S., Obidina Ju V., Ivanova Ju S., Zenin V.V., Shatrova A.N., Borodkina A.V., Aksenov N.D., Zemelko V.I., Burova E.B., Puzanov M.V., Nikolsky N.N.
- Журнал: Oxidative Medicine and Cellular Longevity
- Том: 2015
- Год издания: 2015
- Издательство: Landes Bioscience
- Местоположение издательства: United States
- Первая страница: 502105
- Последняя страница: 502105
- Аннотация: The present study focuses on the involvement of reactive oxygen species (ROS) in the process of mesenchymal stem cells “waking up” and entering the cell cycle after the quiescence. Using human endometrial mesenchymal stem cells (eMSCs), we showed that intracellular basal ROS level is positively correlated with the proliferative status of the cell cultures. Our experiments with the eMSCs synchronized in the G0 phase of the cell cycle revealed a transient increase in the ROS level upon the quiescence exit after stimulation of the cell proliferation. This increase was registered before the entry to the S-phase of the cell cycle and elimination of this increase by antioxidants (N-acetyl-L-cysteine, tempol and resveratrol) blocked G1–S phase transition in eMSCs. Similarly, a cell cycle arrest which resulted from the antioxidant treatment was observed in the experiments with synchronized human mesenchymal stem cells derived from the adipose tissue. Thus, we showed that physiologically relevant level of ROS is required for the initiation of human mesenchymal stem cell proliferation and that low levels of ROS due to the antioxidant treatment can block the stem cell self-renewal.
- Добавил в систему: Бурова Елена Борисовна