Metagenomics revealed a new genus 'Candidatus Thiocaldithrix dubininis' gen. nov., sp. nov. and a new species 'Candidatus Thiothrix putei' sp. nov. in the family Thiotrichaceae, some members of which have traits of both Na+- and H+-motive energetics †

Ravin, N.V.; Muntyan, M.S.; Smolyakov, D.D.; Rudenko, T.S.; Beletsky, A.V.; Mardanov, A.V.; Grabovich, M.Y.

Авторы: Ravin N.V., Muntyan M.S., Smolyakov D.D., Rudenko T.S., Beletsky A.V., Mardanov A.V., Grabovich M.Y.
Журнал: International Journal of Molecular Sciences
Том: 24
Номер: 18
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 1
Последняя страница: 20
Номер статьи: 14199
DOI: 10.3390/ijms241814199
Аннотация: † The study is dedicated to the memory of the outstanding bioenergeticist Prof. V.P. Skulachev. Metagenomics revealed a new genus 'Candidatus Thiocaldithrix dubininis' gen. nov., sp. nov. and a new species 'Candidatus Thiothrix putei' sp. nov. in the family Thiotrichaceae, some members of which have traits of both Na+- and H+-motive energetics. Two metagenome-assembled genomes (MAGs), GKL-01 and GKL-02, related to the family Thiotrichaceae have been assembled from the metagenome of bacterial mat obtained from a sulfide-rich thermal spring in the North Caucasus. Based on average amino acid identity (AAI) values and genome-based phylogeny, MAG GKL-01 represented a new genus within the Thiotrichaceae family. The GC content of the GKL-01 DNA (44%) differed significantly from that of other known members of the genus Thiothrix (50.1–55.6%). We proposed to assign GKL-01 to a new species and genus ‘Candidatus Thiocaldithrix dubininis’ gen. nov., sp. nov. GKL-01. The phylogenetic analysis and estimated distances between MAG GKL-02 and the genomes of the previously described species of the genus Thiothrix allowed assigning GKL-02 to a new species with the proposed name ‘Candidatus Thiothrix putei’ sp. nov. GKL-02 within the genus Thiothrix. Genome data first revealed the presence of both Na+-ATPases and H+-ATPases in several Thiothrix species. According to genomic analysis, bacteria GKL-01 and GKL-02 are metabolically versatile facultative aerobes capable of growing either chemolithoautotrophically or chemolithoheterotrophically in the presence of hydrogen sulfide and/or thiosulfate or chemoorganoheterotrophically.
Добавил в систему: Мунтян Мария Сергеевна

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